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serca2a  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc serca2a
    Serca2a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/serca2a/pm41707812-100-23-24?v=Cell+Signaling+Technology+Inc
    Average 94 stars, based on 82 article reviews
    serca2a - by Bioz Stars, 2026-07
    94/100 stars

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    (A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with 0 mM NAM (control) or 100 mM NAM-supplemented buffer. (B) Representative electrocardiogram (ECG) shows that acute perfusion with 100 mM NAM rapidly induced VF, contractile dysfunction, and eventually led to cardiac arrest (n=4 males, 2 females). (C) Representiative blots and (D) quantification of acetylated <t>SERCA2a</t> levels of left venticular tissue from control and 100 mM NAM-perfused ex vivo mouse hearts (n=3). Data are expressed as mean ± standard error of the mean. Two tailed unpaired Student t-test was used.
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    (A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with 0 mM NAM (control) or 100 mM NAM-supplemented buffer. (B) Representative electrocardiogram (ECG) shows that acute perfusion with 100 mM NAM rapidly induced VF, contractile dysfunction, and eventually led to cardiac arrest (n=4 males, 2 females). (C) Representiative blots and (D) quantification of acetylated SERCA2a levels of left venticular tissue from control and 100 mM NAM-perfused ex vivo mouse hearts (n=3). Data are expressed as mean ± standard error of the mean. Two tailed unpaired Student t-test was used.

    Journal: bioRxiv

    Article Title: Dual effects of nicotinamide on aging-related arrhythmia: protective at low dose, proarrhythmic at high doses

    doi: 10.1101/2025.09.22.677785

    Figure Lengend Snippet: (A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with 0 mM NAM (control) or 100 mM NAM-supplemented buffer. (B) Representative electrocardiogram (ECG) shows that acute perfusion with 100 mM NAM rapidly induced VF, contractile dysfunction, and eventually led to cardiac arrest (n=4 males, 2 females). (C) Representiative blots and (D) quantification of acetylated SERCA2a levels of left venticular tissue from control and 100 mM NAM-perfused ex vivo mouse hearts (n=3). Data are expressed as mean ± standard error of the mean. Two tailed unpaired Student t-test was used.

    Article Snippet: The membrane was then blocked with Intercept Blocking Buffer (927-60001, LI-COR) for 1 h at RT, and incubated with primary antibodies against SERCA2a (1:1000 dilution, 4388, cell signaling), and Pan Acetylation (1:2000 dilution, 66289-1-Ig, Proteintech) overnight at 4°C.

    Techniques: Ex Vivo, Control, Two Tailed Test